Abstract: Interactive learning has become the research focusing on the area of regional innovation. The conception and meaning of interactive learning are elaborated, furthemore the conceptual framework and components of enterprise’s interactive learning in regional innovation are put forward. In addition, the methodology of confirmatory factor analysis and high-order factor analysis are adopted to verify the structure and components of interactive learning based on the consolidated data from a questionnaire survey. It could be used as a source of empirical evidence and theoretical proof for further research on the interactive learning activity in regional innovation.