Abstract: According to the current status of E-commerce development, the E-commerce development stages is classified, a brief review on the main factors which influence the enterprise information technology (including E-commerce) applied behavior is made, through empirical testing in Guangdong province and Guangzhou, Shenzhen, Meizhou, Shaoguan four cities, which are in various E-commerce application stage of agricultural businesss, the potential benefits, competition within industries, ease of information acquisition, conveniences of services, transaction security, and other factors influencing on the application of E-commerce are studied. The results show that the potential benefits, competition within industries, ease of information acquisition, conveniences of services, transaction security influence on the application of E-commerce in different extent and direction.