Abstract: On the basis of dynamic trust theory, a staggered theoretic model which describes the dynamic collaboration trust in supply chain from the point of collaboration relationship is proposed, the dynamic dissimilar effect of specific asset investment on collaboration trust and cooperation intention in differential phase are studied. Forty three supply chain companies in different fields are chosen as the objects of experimental research and the sample data of 318 actual members are finally collected. Factor analysis and structural equation modeling are implemented to validate the hypothesized relationship between specific asset investment, supply chain collaboration trust, and cooperation intention in the theoretic model.