Abstract： Based on the triple helix theory, starting from the concept of Triple Helix Interface Organization (THIO), structural forms of bilateral THIO and trilateral THIO are presented. Moreover， the essence of innovation and the features of organization structure of THIO are explored, the impact of THIO on innovation mechanism are specitically analyzed from several aspects, namely, attributes of carrier and centre， function of coordination, digestion of conflict relationship, decision-making for cooperative solution, and latent function.