The influences of managerial culture on employee's work motivations are regarded widely, but there isn't an exercisable framework on relations between culture and work motivations till now. In this paper, the relations between culture and work motivations are analyzed from the angle of view of the influences of culture dimensions on employee's perceptions on incentive factors, by using Hofstede's culture dimension theory and summing up of work incentive factors, the author chosed Wenzhou region in Zhejiang province and Guanzhong region in Shanxi province as two typical culture areas, surveyed on 412 employees in 21 enterprises, and got data of five culture dimensions and five incentive factors perception. And then, the author analyzed the influences of culture dimensions on incentive factors perception dimensions and got a framework of the influences of managerial culture on employee's work motivations.