After joined the charitable organizations, people with caring heart found that the reality is inconsistent with the expected work or organizational status, thus resulting in higher turnover intention and lower well-being and organizational citizenship behavior, causing inefficient management of those organizations. In order to analyze the causes of this phenomenon, this study focuses on psychological contract violation and analyzes the mechanism from the perspective of the workplace spirituality. Using structural equation modeling (SEM), this study found those results in charitable organizations:(1) Psychological contract violation negatively affects employees' subjective well-being and organizational citizenship behavior, and positively affects employees' turnover intention; (2) Psychological contract violation negatively affects employees' workplace spirituality; (3) Workplace spirituality plays a partial intermediary role between psychological contract violation and subjective well-being. Workplace spirituality plays a complete intermediary role between psychological contract violation and organizational citizenship behavior, as well as psychological contract violation and turnover intention.