Incorporated the case study and literature, the paper constructed a two-order factor model of innovative culture evaluating construction. One-order contained nine factors:work environment, condition, encouragement system, development of institute, leadership behavior, values, self-confidence, consciousness of collaboration, innovative atmosphere. Two-order constructed of three factors: environment and condition, system and behavior, values and beliefs. Questionnaire survey on the internet inquired 5678 subjects who came from 81 research institutes of Chinese Academy of Sciences. The findings indicated the statistic data supported the innovative culture evaluating construction, the fit indices were perfect. Both reliability and validity of the innovative culture questionnaire achieved the requirement of psychometrics. This model got the actual value for CAS to evaluated the innovative culture construction,it also could be used as the guideline of innovative culture evaluating construction in other research institutes.